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Sysmex Corporation diff quik staining method
Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics <t>using</t> <t>Diff-Quik</t> staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.
Diff Quik Staining Method, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-Pollutants in a Murine Model of Allergic Rhinitis"

Article Title: Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-Pollutants in a Murine Model of Allergic Rhinitis

Journal: Clinical and Experimental Otorhinolaryngology

doi: 10.21053/ceo.2025-00170

Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.
Figure Legend Snippet: Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

Techniques Used: Diff-Quik, Staining, Standard Deviation

Related Articles

Staining:

Article Title: Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-Pollutants in a Murine Model of Allergic Rhinitis
Article Snippet: .. Slides were stained using the Diff-Quik staining method (Sysmex). ..

Article Title: Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-pollutants in a Murine Model of Allergic Rhinitis.
Article Snippet: .. Slides were stained using the Diff-Quik staining method (Sysmex, Kobe, Japan). ..

Diff-Quik:

Article Title: Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-Pollutants in a Murine Model of Allergic Rhinitis
Article Snippet: .. Slides were stained using the Diff-Quik staining method (Sysmex). ..

Article Title: Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-pollutants in a Murine Model of Allergic Rhinitis.
Article Snippet: .. Slides were stained using the Diff-Quik staining method (Sysmex, Kobe, Japan). ..

other:

Article Title: Comparison of dose-response relations between 4-week inhalation and intratracheal instillation of NiO nanoparticles using polimorphonuclear neutrophils in bronchoalveolar lavage fluid as a biomarker of pulmonary inflammation.
Article Snippet: Inhalation studies and intratracheal instillation studies using laboratory animals are commonly conducted for pulmonary toxicity tests of nanomaterials.. In our study, male Wister rats were exposed to nickel oxide (NiO) particles including a nano-scale, even for aerosols and suspensions, in a 4-week inhalation and intratracheal instillation.. Using polymorphonuclear neutrophils (PMNs) in bronchoalveolar lavage fluid as a biomarker of inflammation, we attempted to quantify the relationship between responses to inhalation and intratracheal instillation of the nanoparticles, based on surface area doses.

Article Title: Inhalation of titanium dioxide (P25) nanoparticles to rats and changes in surfactant protein (SP-D) levels in bronchoalveolar lavage fluid and serum.
Article Snippet: Titanium dioxide (TiO2) nanoparticles are typical and widely used nanomaterials, and there are many studies on the inflammatory responses induced by their inhalation.. In this study, we conducted a 4-week inhalation exposure study of aerosolized TiO2> nanoparticles (P25) to male Wistar rats.. The mean aerosol concentration measured at each day was 4.1mg/m by dry powder dispersion of TiO2 nanoparticles.

Article Title: Rapid on-site evaluation by endosonographer during endoscopic ultrasound-guided fine needle aspiration for pancreatic solid masses.
Article Snippet: Background and Aim: Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is an established diagnostic method for patients with suspected pancreatic ductal carcinoma.. Rapid on-site evaluation (ROSE) has been reported to improve the accuracy.. However, an on-site cytopathologist is not routinely available in many institutions.

Article Title: Changes over time in pulmonary inflammatory response in rat lungs after intratracheal instillation of nickel oxide nanoparticles
Article Snippet: Smears from the above cellular sample were prepared on glass slides by the Cytospin method and stained by a Diff‐Quik kit (Sysmex Co., Kobe, Japan) to measure the ratio of PMN in total cell.

Article Title: Pulmonary responses in rat lungs after intratracheal instillation of 4 crystal forms of titanium dioxide nanoparticles
Article Snippet: From the above samples, smears were prepared on glass slides by the CytoSpin method and stained by a Diff-Quik kit (Sysmex Co., Kobe, Japan).



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Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

Journal: Clinical and Experimental Otorhinolaryngology

Article Title: Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-Pollutants in a Murine Model of Allergic Rhinitis

doi: 10.21053/ceo.2025-00170

Figure Lengend Snippet: Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

Article Snippet: Slides were stained using the Diff-Quik staining method (Sysmex).

Techniques: Diff-Quik, Staining, Standard Deviation